电针、敷脐疗法联合 HCG 治疗黄素化未破裂卵泡综合征临床研究

目的:观察电针、敷脐疗法联合人绒毛膜促性腺激素(HCG)治疗对黄素化未破裂卵泡综合征(LUFS)患者血清雌二醇(E2)、黄体生成素(LH)、排卵率及妊娠率的影响。方法:将60例LUFS患者按随机数字表法分为观察组和对照组各30例。对照组单纯给予HCG治疗,观察组给予电针、敷脐疗法联合HCG治疗,2组均连续治疗3个月经周期。观察2组治疗前后血清E2、LH含量的变化及排卵、妊娠情况。结果:治疗后,2组LH、E2水平均较治疗前升高,观察组LH、E2水平均高于对照组,差异均有统计学意义(P<0.05)。观察组排卵率、妊娠率均高于对照组,差异均有统计学意义(P<0.05)。结论:电针、敷脐疗法联合HCG治疗LUFS,可以提升LH、E2水平,促进排卵,提高妊娠率。     

补肾凉血安胎法治疗肾虚血热型先兆流产合并官腔积液临床研究

目的:研究补肾凉血安胎法治疗肾虚血热型先兆流产合并宫腔积液的效果。方法:选择肾虚血热型先兆流产合并宫腔积液患者160例,按随机数字表法分为对照组和观察组各80例。对照组给予黄体酮联合酚磺乙胺治疗,观察组采用补肾凉血安胎法治疗,连续治疗2周。观察2组临床疗效,比较2组孕酮(P)、雌二醇(E2)、人绒毛膜促性腺激素(HCG),以及中医症候评分。结果:观察组总有效率为85.00%,高于对照组70.00%(P<0.05)。治疗前,2组P、HCG、E2水平比较,差异无统计学意义(P>0.05)。治疗后,2组P、HCG、E2水平较治疗前升高(P<0.05),且观察组P、HCG、E2水平高于对照组(P<0.05)。治疗前,2组各项中医症状评分比较,差异无统计学意义(P>0.05)。治疗后,2组各项中医症状评分较治疗前降低(P<0.05),且观察组各项中医症状评分低于对照组(P<0.05)。结论:采用补肾凉血安胎汤治疗肾虚血热型先兆流产合并宫腔积液患者,不仅能改善患者血液指标,有利于保胎,还能促进宫腔积液的吸收,效果显著,值得临床应用。     

Halogenated benzoate derivatives of altholactone with improved anti-fungal activity

Altholactone exhibited the anti-fungal activity with a high MIC value of 128 μg ml^?1 against Cryptococcus neoformans and Saccharomyces cerevisiae. Fifteen ester derivatives of altholactone 1–15 were modified by esterification and their structures were confirmed by spectroscopic methods. Most of the ester derivatives exhibited stronger anti-fungal activities than that of the precursor altholactone. 3-Bromo- and 2,4-dichlorobenzoates (7 and 15) exhibited the lowest minimal inhibitory concentration (MIC) values against C. neoformans at 16 μg ml^?1_, while the 4-bromo-, 4-iodo-, and 1-bromo-3-chlorobenzoates (11–13) displayed potent activity against S. cerevisiae with MIC values of 1 μg ml^?1. In conclusion, this analysis indicates that the anti-fungal activity of altholactone is enhanced by addition of halogenated benzoyl group to the 3-OH group.     

Turner Syndrome and Fertility

Turner syndrome (TS) is tightly associated with hypergonadotropic hypogonadism and ovarian dysgenesis, typically resulting in infertility in the great majority of patients. Therefore females with TS are usually treated with female sex steroids from 11–12 years of age until the normal age of natural menopause of around 53–54 years of age. Infertility is rated among females with TS as a distressing concern and a detractor from a good quality of life. Options for motherhood for females with TS has expanded during recent years. Originally, only adoption was an option, unless of course for the small minority of TS females that still has ovarian function and are capable of achieving pregnancy through normal means. Oocyte donation has become the mainstream option in many countries and seems to work well, especially if patients have been treated with optimal estrogen and gestagen for a prolonged time before the intervention. It comes with an increased risk of cardiovascular complications and TS oocyte donation pregnancies are viewed as high risk pregnancies necessitating increased vigilance. Oocyte cryopreservation of own oocytes is also becoming an option in a select group of TS and has special challenges. Ovarian tissue cryopreservation is a promising new techniques that has been applied successfully in children with cancer. Currently, several trials are running around the world evaluating this techniques in TS. The genetics and genomics behind the ovarian dysgenesis seen in TS is not understood, but new studies have elucidated global changes in DNA methylation and RNA expression in blood from persons with TS and it is likely that similar changes are present in the ovaries. We still, however, need more thorough research to fully uncover the genetic background of ovarian failure in TS. Gene expression studies and methylation analysis from ovarian TS tissues still needs to be performed.     

雌二醇、孕酮及β-人绒毛膜促性腺激素水平变化对早期先兆性流产的预测价值分析

目的:研究雌二醇(E_2)、孕酮(P)及β-人绒毛膜促性腺激素(β-HCG)水平变化对早期先兆性流产的预测价值。方法:选择2014年12月至2015年10月在我院就诊的早期妊娠女性130例,按照临床检查情况分为A组及B组,A组均为早期先兆性流产妊娠女性,B组均为早期输卵管妊娠女性;再选择正常早孕孕妇65例纳入C组,三组一般资料比较无统计学意义(P0.05)。三组均于入院后次日及第8d行E_2、P、β-HCG检测,比较三组检测结果及诊断符合率。结果:C组E_2、P、β-HCG检测值均显著高于A组、B组(P均0.05);A组E_2、P、β-HCG检测值均显著高于B组(P均0.05);入院8d后A组E_2检测值较入院次日显著降低(P0.05),P检测值降低幅度较小(P0.05),β-HCG检测值较入院次日显著升高(P0.05);8d后B组E_2、P、β-HCG检测值均较入院次日无显著变化(P均0.05);8d后C组E_2、P、β-HCG检测值均较入院次日显著升高(P均0.05)。联合诊断符合率(95.00%)远高于E_2(83.33%)、P(81.67%)、β-HCG(78.33%),P均0.05;结论:E_2、P、β-HCG联合检测对先兆流产较E_2、P、β-HCG单独检测具有更高的应用价值。     

九里香叶对去卵巢大鼠拟雌激素样作用的实验研究

目的探讨九里香叶对去卵巢大鼠的拟雌激素样作用。方法以2%戊巴比妥钠麻醉大鼠,切除大鼠双侧卵巢,对照大鼠切除卵巢旁同样大小的脂肪团。术后7天分组、给药。分为模型组:生理盐水(0.4 mg/kg/d);对照组:生理盐水(0.4 mg/kg/d);戊酸雌二醇组:0.4 m g/kg/d;九里香高剂量组:200 mg/kg/d;九里香中剂量组:100 mg/kg/d;九里香低剂量组:50 mg/kg/d。连续给药90天后,处死大鼠,测定子宫系数。放射免疫法测定血清雌二醇水平。结果给药第30天、60天、90天,与对照组比较,模型组大鼠体质量增幅较大,差异显著;与模型组比较,戊酸雌二醇组、九里香叶高剂量组大鼠体质量增幅较小,差异显著(P0.05)。与模型组比较,戊酸雌二醇组、九里香叶高剂量组大鼠子宫质量和子宫系数明显增加,差异显著(P0.05)。与模型组比较,戊酸雌二醇组、九里香叶高、中、低剂量组大鼠雌二醇明显增加,差异不显著(P0.05)。结论九里香叶具有一定的雌激素样作用。     

Lip impressions: a new method for monitoring morphological changes in orofacial granulomatosis

AIM: To develop and evaluate an objective method for assessing lip size and treatment-related morphological changes in orofacial granulomatosis (OFG) patients. MATERIALS AND METHODS: Patients with swollen lips because of OFG (n=21) were enrolled. A light-body polyvinylsiloxane material was used to take lip impressions before and after treatment (n=10), or during treatment (n=11). Plaster models were cast from the impressions and the lips were measured using callipers. The intra-examiner and inter-examiner reproducibility of the technique were assessed. RESULTS: OFG patients had significantly larger lips than controls (P<0.0001). The coefficient of variation on repeated measurements of the same impression was 1.6% and for duplicate impressions was 2.6%. Significant reduction in lip size was shown in all 10 patients after diet restriction (P<0.002). Seven of 11 patients whose impressions were taken at least 3 months after the initiation of cinnamon- and benzoate-free diet also showed reduction in lip size during follow up (P<0.002). CONCLUSIONS: Serial lip impressions appear to be reliable for routine quantification of morphological changes of the lips in OFG patients. We present a new reproducible and sensitive method for assessing changes in lip size in response to treatment in OFG.     

口腔颌面部血管瘤激素治疗的疗效评价

国内外应用类固醇激素治疗口腔颌面部血管瘤疗效报道不一致,在很大程度上与传统分类方法紊乱有关。本研究采用血管瘤和血管畸形的新分类,对31例血管瘤进行口服强的松治疗。根据服药后病变改变大小、颜色深浅变化将疗效分为优、良、中、差。服药前采用放射免疫方法进行血清Es水平测定,来探讨其疗效与血清E2之间的关系。结果表明:口服强的松治疗血管瘤的有效率为74％。强的松疗效优、良、中、差各组间的血清E2水平无显著差异(P>0.05),说明强的松疗效好坏与血清E2水平无关。     

IVF周期中高雌、孕激素水平对着床期子宫整合素αvβ3表达及子宫内膜容受性的影响

目的 探讨IVF超促排卵过程中,超生理剂量的雌、孕激素对着床期整合素αvβ3表达的影响,从而推测IVF超促排卵所致的高雌、孕激素对子宫内膜容受性的影响.方法 2012年5月至2013年10月于云南省第一人民医院生殖—科行IVF的33例因卵巢过度刺激综合征或过高孕酮水平取消新鲜周期胚胎移植的不孕症妇女为研究组,根据HCG日雌孕激素水平将研究对象分为3组:高E2组(E2≥5 210 pg/mL、P＜ 1.05 ng/mL);高P组(P≥1.05 ng/mL、E2＜5 210 pg/mL);高E2并高P组(P≥1.05 ng/mL、E2≥5 210 pg/mL),于取卵后7～8d用宫腔负压吸管(Pipe11e管)取内膜组织.对照组为11例正常妇女,于超声监测排卵后7～8 d同法取内膜,采用免疫组化SP法和组织学积分H-score法对整合素α v β 3在种植窗期子宫内膜中的表达进行定位和半定量分析,比较不同雌孕激素水平在着床期对整合素αvβ3表达的影响,从而推测IVF超促排卵中高雌、孕激素对子宫内膜容受性的影响.结果 种植窗期高E2组、高P组、高E2并高P组患者与对照组比较整合素α vβ 3的表达差异没有统计学意义(P＞0.05).结论 仅仅依靠整合素αvβ3的表达来推测子宫内膜的容受性是不可靠的.